目
录
中文摘要 .....................................................................................................................Ⅰ ABSTRACT ................................................................................................................Ⅱ 前
言 ...........................................................................................................................1 第一部分 益肾克癃方抗实验性小鼠前列腺增生作用的研究 .................................3
一、材料和方法 ............................................................................................................................3
（一）实验动物 .......................................................................................................................3
（二）药材与化学试剂 .........................................................................................................3
（三）实验仪器 .......................................................................................................................3
（四）实验方法 .......................................................................................................................4 1 药物的制备 ...................................................................................................4 2 动物分组、造模和给药 ...............................................................................4 3 观察指标及测定方法 ...................................................................................4 4 统计学处理 ...................................................................................................5
二、结果 ..........................................................................................................................................5
（一）小鼠的一般情况 .........................................................................................................5
（二）对小鼠前列腺湿重及前列腺指数的影响 .........................................................5
（三）对小鼠血清中酸性磷酸酶水平的影响 ..............................................................6
三、分析与讨论 ............................................................................................................................6
四、小结 ..........................................................................................................................................7 第二部分 益肾克癃方抗去势大鼠前列腺增生作用及其作用机制初步研究 ..........8 实验一 益肾克癃方抗去势大鼠前列腺增生作用研究 ..............................................8
一、材料与方法 ............................................................................................................................8
（一）实验动物 .......................................................................................................................8
（二）药材与化学试剂 .........................................................................................................8
（三）实验仪器 .......................................................................................................................9
（四）实验方法 .......................................................................................................................9 1 药物的制备 ...................................................................................................9 2 动物分组、造模和给药 ...............................................................................9
3 实验评价指标及测定方法 .........................................................................10 4 统计学处理 .................................................................................................................................11
二、结果 .......................................................................................................................................11
（一）大鼠的一般情况 ......................................................................................................11
（二）对去势大鼠前列腺湿重及前列腺指数的影响 ............................................11
（三）对去势大鼠血清中总酸性磷酸酶水平和前列腺特异性酸性磷酸酶水平的影响 .......................................................................................................................................11
（四）对去势大鼠前列腺组织切片光镜下形态学的影响 ...................................12
（五）对去势大鼠腺体上皮细胞高度及腺腔褶皱指数的影响 .........................13
三、分析与讨论 .........................................................................................................................13
（一）前列腺增生模型的选择 .......................................................................................13
（二）对酸性磷酸酶和前列腺特异性酸性磷酸酶的影响 ...................................14
（三）对前列腺组织的影响 ............................................................................................14
四、小结 .......................................................................................................................................15 实验二 益肾克癃方对去势大鼠前列腺增生作用机制及并发症相关指标的初步研究 .................................................................................................................................16
一、材料与方法 .........................................................................................................................16
（一）实验动物 ....................................................................................................................16
（二）药材与化学试剂 ......................................................................................................16
（三）实验仪器 ....................................................................................................................16
（四）实验方法 ....................................................................................................................16 1 药物的制备 .................................................................................................16 2 动物分组、造模和给药 .............................................................................16 3 实验评价指标及测定方法 .........................................................................17 4 统计学处理 .................................................................................................................................20
二、结果 .......................................................................................................................................21
（一）对去势大鼠血清中睾酮、雌二醇的影响 ......................................................21
（二）对去势大鼠前列腺组织中丙二醛和一氧化氮含量的影响 .....................21
（三）对去势大鼠精囊腺湿重及其指数的影响 ......................................................22
（四）对去势大鼠肾脏指数和肝脏指数的影响 ......................................................23
（五）对去势大鼠肾脏组织形态学的影响................................................................23
三、分析与讨论 .........................................................................................................................23
（一）前列腺增生的作用机制 .......................................................................................23
（二）对性激素的影响 ......................................................................................................24
（三）对一氧化氮和丙二醛的影响 ..............................................................................25
（三）对精囊腺、肾脏、肝脏的影响 .........................................................................25
四、小结 .......................................................................................................................................26 第三部分 益肾克癃方抗实验性大鼠前列腺增生作用及其作用机制初步研究 ....27 实验一 益肾克癃方抗实验性大鼠前列腺增生作用的研究 ....................................27
一、材料与方法 .........................................................................................................................27
（一）实验动物 ....................................................................................................................27
（二）药材与化学试剂 ......................................................................................................27
（三）实验仪器 ....................................................................................................................27
（四）实验方法 ....................................................................................................................27 1 药物的制备 .................................................................................................27 2 动物分组、造模和给药 .............................................................................27 3 实验评价指标及测定方法 .........................................................................28 4 统计学处理 .................................................................................................................................28
二、结果 .......................................................................................................................................29
（一）大鼠的一般情况 ......................................................................................................29
（二）对大鼠前列腺湿重及前列腺指数的影响 ......................................................29
（三）对大鼠血清中总酸性磷酸酶水平和前列腺特异性酸性磷酸酶水平的影响 ................................................................................................................................................29
（四）对大鼠前列腺组织切片光镜下形态学的影响 ............................................30
（五）对大鼠腺体上皮细胞高度及腺腔褶皱指数的影响 ...................................31
三、分析与讨论 .........................................................................................................................32
（一）关于动物模型的复制与评价 ..............................................................................32
（二）抗实验性大鼠前列腺增生作用 .........................................................................33
四、小结 .......................................................................................................................................34 实验二 益肾克癃方抗实验性大鼠前列腺增生作用机制及并发症相关指标的初步研究 .............................................................................................................................35
一、材料与方法 .........................................................................................................................35
（一）实验动物 ....................................................................................................................35
（二）药材与化学试剂 ......................................................................................................35
（三）实验仪器 ....................................................................................................................35
（四）实验方法 ....................................................................................................................35 1 药物的制备 .................................................................................................35 2 动物分组、造模和给药 .............................................................................35 3 实验评价指标及测定方法 .........................................................................35 4 统计学处理 .................................................................................................................................36
二、结果 .......................................................................................................................................36
（一）对大鼠血清中睾酮、雌二醇的影响................................................................36
（二）对大鼠前列腺组织中丙二醛和一氧化氮含量的影响 ..............................37
（三）对大鼠精囊腺湿重及其指数的影响................................................................38
（四）对大鼠肾脏指数和肝脏指数的影响................................................................38
（五）对大鼠肾脏组织形态学的影响 .........................................................................38
三、分析与讨论 .........................................................................................................................39
（一）对性激素水平的影响 ............................................................................................39
（二）雌激素在BPH中的作用 .....................................................................................40
（三）对NO和MDA的影响 ........................................................................................40
（三）对精囊腺、肾脏、肝脏的影响 .........................................................................41
四、小结 .......................................................................................................................................41 结论 .....................................................................................................................................................42 参考文献 ............................................................................................................................................43 附图 .....................................................................................................................................................47 致谢 .....................................................................................................................................................51 文献综述 ............................................................................................................................................52
